The Landmark on Film: Representations of Place and Identity

This paper examines two documentary essays focusing on landmark architecture in the transnational Øresund region comprising Copenhagen and Malmö. I argue that the motif of construction and deconstruction is congruous to our understanding of the ways identities are negotiated vis-à-vis spatial experience. In the lms, the multiple trajectories of characters of diverse nationalities and cultures are woven into the (de)construction of the landmark structures, producing a visual space that interrogates what ‘identity’ means in an increasingly networked and global world

Regional screen ecosystems at the peripheries: Production and talent development in Tromsø and Aarhus

This article addresses the regionalisation of screen culture in Norway and Denmark, focusing on how regional screen entities in Tromsø and Aarhus are working to professionalise production and talent development at the peripheries of both countries. We outline their distinctive characteristics and circumstances as regional hubs and delineate the key actors that constitute the respective screen ecosystems. We analyse the interplay between regional film policy, production, and talent development in relation to regional development, geography, creativity, innovation, and the economy of culture. Based on an analysis of policies, strategy documents, and interviews conducted with practitioners in Aarhus and Tromsø over the period 2014–2019, we explore the diverse strategies that these regional production hubs employ to develop and—more challengingly—retain talents in the region, and argue that despite the increased attention given to ‘diversity’ in film policy, structural and cultural obstacles remain in the way of sustainable growth

Losing Control: Lars von Trier and the Production of Authenticity and the Auteur

Master'sMASTER OF ART

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Exploring the Root Causes of Human Error in Small, Medium Enterprise (SMEs) Manufacturing Industries in Malaysia

The SMEs manufacturing industry can be considered as the country’s backbone which helps to develop Malaysia’s economy. However, the greater the development of manufacturing, the greater of workplace accidents and injuries. Human errors are the foundation of all causes of occupational accidents and injuries. The objective of this research is to identify the main causes of human error in SMEs manufacturing industry by using quantitative research approach. This study revealed that factors such as mental workload and working condition has significant relationship with human error. However, variable such as horseplay or, prank at work as well as working duration (shift work) do not show any significant relationship with human error. This study ended with a discussion and interpretation of the research findings and provides contribution to manufacturing industry, Human Resource practitioner or policy maker, as well as the employee in preventing error

Antibacterial activities of mechanochemically synthesized perovskite strontium titanate ferrite metal oxide

This work explored strontium titanate ferrite (SrTi1−xFexO3−δ or STFx in short) metal oxide as an effective antibacterial agent and investigated its bactericidal mechanism. The perovskite STFx nanoparticles (x = 0, 0.2, 0.4, 0.6, 0.8 and 1) were successfully synthesized with high energy ball milling approach. The feasibility of utilizing this material for antibacterial application was studied on Escherichia coli (E. coli) in the presence of dispersed STF0.8 nanoparticles in water. Excellent bactericidal effect has been observed by killing all the E. coli (∼105 CFU/mL) within 15 min in both light and dark conditions, excluding photocatalysis as the major contributing mean of bactericidal effect. Mechanism study via surface charge characterization, fluorescence microscope observation, inductively coupled plasma measurement and SEM examination has revealed that the positive surface charge, high pH environment, Sr2+ dissociation and nano-size of STF0.8 metal oxide could have collectively contributed to its excellent bactericidal effect. These results have increased the potential to apply STFx in water purification for microorganism destruction.ASTAR (Agency for Sci., Tech. and Research, S’pore

Evaluation of NG-Test CARBA 5 version 2, Cepheid Xpert Carba-R, and carbapenem inactivation methods in comparison to whole-genome sequencing for the identification of carbapenemases in non-fermenting Gram-negative bacilli

NG-Test CARBA 5 (NG-Biotech) is a rapid in vitro multiplex immunoassay for the phenotypic detection and differentiation of the "big five" carbapenemase families (KPC, OXA-48-like, VIM, IMP, and NDM). Version 2 of this assay was evaluated alongside the Xpert Carba-R assay (Cepheid, Inc.), the modified carbapenem inactivation method (mCIM), and the CIMTris assay, with a collection of carbapenem-resistant non-fermenting Gram-negative bacilli comprising 138 Pseudomonas aeruginosa and 97 Acinetobacter baumannii isolates. Whole-genome sequencing (WGS) was used as the reference standard. For P. aeruginosa, NG-Test CARBA 5 produced an overall percentage agreement (OPA) with WGS of 97.1%, compared with 92.8% forXpert Carba-R and 90.6% for mCIM. For A. baumannii, as OXA-type carbapenemases (non-OXA-48) are not included, both the NG-Test CARBA 5 and Xpert Carba-R only had an OPA of 6.2%, while the CIMTris performed well with an OPA of 99.0%. The majority of A. baumannii isolates (95.9%) tested falsely positive for IMP on NG-Test CARBA 5; no IMP genes were found on WGS. No clear cause was found for this phenomenon; a cross-reacting protein antigen unique to A. baumannii is a possible culprit. NG-Test CARBA 5 performed well for carbapenemase detection in P. aeruginosa. However, results from A. baumannii isolates should be interpreted with caution.Ministry of Health (MOH)National Medical Research Council (NMRC)Published versionThis work was supported by the Ministry of Health, Singapore, under the FY16 Health Service Development Programme (HSDP) Project 19N01: "Reducing the spread of carbapenemase producing Gram-negative bacteria via rapid and direct detection from surveillance and clinical samples" (S.V.), as well as the National Medical Research Council (NMRC) Centre Grant: “Collaborative Solutions Targeting Antimicrobial Resistance Threats in Health Systems (CoSTAR-HS)" (CG21APR2005), NMRC Clinician Scientist Award (MOH-000276), NMRC Open Fund—Large Collaborative Grant: AntiMicrobial resistance Research & Intervention Alliance Singapore (AMRITAS; MOH-001326-01) (O.T.N.), and NMRC Clinician Scientist Individual Research Grant (CIRG18Nov-0034) (K.M.)